| Seth MacFarlane | Peter Griffin/Brian Griffin/Stewie Griffin/Glen Quagmire/Tom Tucker/Additional Voices |
| Alex Borstein | Lois Griffin/Loretta/Tricia Takanawa/Barbara Pewterschmidt/Additional Voices |
| Mila Kunis | Meg Griffin (2000-2002) |
| Seth Green | Chris Griffin/Neil Goldman/Additional Voices |
| Mike Henry | Cleveland/Cleveland Jr. |
| Patrick Warburton | Joe Swanson |
| Butch Hartman | Mr. Jonathan Weed (1999-2001) |
| Lori Alan | Diane Simmons/Additional Voices |
| Carlos Alazraqui | Mr. Jonathan Weed (1999-2000) |
| Adam West | Mayor Adam West (2000-2002) |
| Jennifer Tilly | Bonnie Swanson |
| John G. Brennan | Mort Goldman (2001-2002) |
| Wally Wingert | Additional Voices (1999-2002) |
| Lacey Chabert | Meg Griffin (1999-2000) |
